homocysteine induces heme oxygenase-1 expression via transcription factor nrf2 activation in hepg2 cells
Authors
abstract
background: elevated level of plasma homocysteine has been related to various diseases. patients with hyperhomocysteinemia can develop hepatic steatosis and fibrosis. we hypothesized that oxidative stress induced by homocysteine might play an important role in pathogenesis of liver injury. also, the cellular response designed to combat oxidative stress is primarily controlled by the transcription factor nrf2, a principal inducer of anti-oxidant and phase ii-related genes. methods: hepg2 cells were treated with homocysteine in different time periods. glutathione content was measured by flowcytometry. using electrophoretic mobility shift assay (emsa) and western-blotting, anti-oxidant response element (are)-binding activity of nrf2 for heme ocygenase-1 (ho-1) was demonstrated. real time rt-pcr and western-blotting were performed to evaluate whether homocysteine was able to induce mrna and protein expression of ho-1. results: the role of nrf2 in cellular response to homocysteine is substantiated by the following observations in hepg2 cells exposed to homocysteine (i) western-blotting revealed that nrf2 is strongly stabilized and became detectable in nuclear extracts. (ii) emsa demonstrated increased binding of nrf2 to oligomers containing ho-1 promoter-specific are-binding site. (iii) real time rt-pcr and western-blotting revealed increased mrna and protein expression of inducible gene ho-1 after treatment with homocysteine. conclusion: data presented in the current study provide direct evidence that the immediate cellular response to oxidative stress provoked by homocysteine is orchestrated mainly by the nrf2-are pathway. therefore, induction of nrf2-are-dependent expression of ho-1 could be a therapeutic option for hepatic cells damage induced by homocysteine.
similar resources
Homocysteine Induces Heme Oxygenase-1 Expression via Transcription Factor Nrf2 Activation in HepG2 Cells
Background: Elevated level of plasma homocysteine has been related to various diseases. Patients with hyperhomocysteinemia can develop hepatic steatosis and fibrosis. We hypothesized that oxidative stress induced by homocysteine might play an important role in pathogenesis of liver injury. Also, the cellular response designed to combat oxidative stress is primarily controlled by the transcripti...
full textHomocysteine induces heme oxygenase-1 expression via transcription factor Nrf2 activation in HepG2 cell.
BACKGROUND Elevated level of plasma homocysteine has been related to various diseases. Patients with hyperhomocysteinemia can develop hepatic steatosis and fibrosis. We hypothesized that oxidative stress induced by homocysteine might play an important role in pathogenesis of liver injury. Also, the cellular response designed to combat oxidative stress is primarily controlled by the transcriptio...
full texteckol enhances heme oxygenase-1 expression through activation of Nrf2/JNK pathway in HepG2 cells.
Eckol isolated from Ecklonia stolonifera was previously reported to exhibit cytoprotective activity with its intrinsic antioxidant activity in in vitro studies. In this study, we characterized the mechanism underlying the eckol-mediated the expression of heme oxygenase-1 (HO-1). Eckol suppressed the production of intracellular reactive oxygen species and increased glutathione level in HepG2 cel...
full textZerumbone induces heme oxygenase-1 expression in mouse skin and cultured murine epidermal cells through activation of Nrf2 [Running title: Nrf2-mediated heme oxygenase-1 expression by zerumbone]
College of Pharmacy and WCU Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-742, South Korea, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan, Department of Food and Nutrition, Sungsin Women’s University, Seoul 136-742, South Korea, Graduate School of Bioscience a...
full textMorin Induces Heme Oxygenase-1 via ERK-Nrf2 Signaling Pathway
BACKGROUND Oxidative stress damages to cells or tissues, however, cellular defense systems including heme oxygenase-1 (HO-1) protects them against oxidative stress. Flavonoid compounds can activate cellular defense mechanisms against oxidative stress and it can reduce cell damages. In the present study, the cytoprotective effects of morin (3,5,7,2',4'-pentahydroxyflavone), in terms of HO-1 enzy...
full textDimethoxycurcumin, a Synthetic Curcumin Analogue, Induces Heme Oxygenase-1 Expression through Nrf2 Activation in RAW264.7 Macrophages
Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] induces heme oxygenase-1 (HO-1) expression via activation of the nuclear factor-erythroid-2-related factor 2 (Nrf2), whereas tetrahydrocurcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-3,5-heptanedione], one of curcumin in vivo metabolites, has no effect on HO-1 expression and Nrf2 activation. The aim of this study was to investi...
full textMy Resources
Save resource for easier access later
Journal title:
iranian biomedical journalجلد ۱۷، شماره ۲، صفحات ۹۳-۱۰۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023